HPLC Test (Hb HPLC): Hemoglobin Variant Analysis
The HPLC blood test (High Performance Liquid Chromatography) analyzes hemoglobin fractions in red blood cells. It separates and measures hemoglobin types and variants to support laboratory evaluation of hemoglobinopathies and related blood-disorder workups.

Quick Summary
- Test name: HPLC Test / Hb HPLC (Hemoglobin fraction analysis)
- Sample: Blood
- Preparation: Fasting is not required for Hb HPLC
- Sample collection: Free home/office collection as per the convenience of the beneficiary
- Reporting time: 24-48 hours
- Service availability: India (multiple cities)
Quick facts
What it measures
- Hemoglobin fractions (such as HbA, HbA2, HbF)
- Hemoglobin variants (such as HbS, HbC, HbE) when present
- Chromatogram peaks and retention patterns used in lab interpretation
What you receive
- Quantitative hemoglobin fraction report
- Chromatogram/graph output
- Lab comments when applicable
When it is ordered
- Workup of suspected hemoglobin variants
- Evaluation of anemia with abnormal red cell indices
- Carrier screening and prenatal screening support as advised by a clinician
Preparation
- Fasting is not required for Hb HPLC
- Keep an ID ready for the sample collection visit
- Share prior CBC/iron studies with your clinician for correlation
What’s Included in the HPLC Test
Report components
- Hemoglobin fraction quantification (HbA, HbA2, HbF)
- Variant detection output (HbS/HbC/HbE peaks when present)
- Chromatogram peak pattern summary
- Laboratory comments when applicable
Sample report preview
This is a format preview for understanding how the output is presented.

Preparation for the HPLC Test
- Fasting: Not required
- Sample type: Blood
- Timing: Choose a convenient collection slot
- Documents: Keep previous CBC/iron profile reports (if available) for clinician correlation
Sample Collection
Free home/office collection as per the convenience of the beneficiary
A trained phlebotomist collects the blood sample at the selected address and time slot.
Reporting Time
24-48 hours
Reporting time is counted after the sample is received for processing.
Interpretation and Clinical Correlation
The HPLC test report is a laboratory measurement of hemoglobin fractions and variants. Interpretation is done by a clinician using clinical history and supporting lab results (such as CBC and iron studies).
This page is informational and supports booking; it does not provide diagnosis or treatment advice.
Who May Be Advised to Take the HPLC Test
- Individuals with unexplained anemia or abnormal RBC indices
- Those with family history of hemoglobin disorders
- Couples planning a pregnancy as part of clinician-advised screening
- Patients referred for hemoglobin variant evaluation
Service Coverage in India
You can book the HPLC Test across multiple Indian cities. Coverage includes:
Delhi, Mumbai, Bengaluru, Hyderabad, Chennai, Kolkata, Pune, Ahmedabad, Jaipur, Lucknow, Surat, Bhopal, Patna, Indore, Kanpur, Vadodara, Thane, Visakhapatnam, Nashik, Ludhiana, Faridabad, Agra, Amritsar, Varanasi, Prayagraj, Coimbatore, Madurai, Raipur, Ranchi, Chandigarh, Guwahati, Vijayawada, Gwalior, Jodhpur, Nagpur, Bhubaneswar, Aurangabad, Meerut, Noida, Ghaziabad, Dhanbad, Kota, Solapur, Bareilly, Jabalpur, Hubli, Mangalore, Mysuru, Dehradun, Navi Mumbai, Jamshedpur
How to Book the HPLC Test
- Open the HPLC Test booking section on this page.
- Select a preferred date and time slot for sample collection.
- Enter beneficiary details and the collection address.
- Confirm the order and complete the booking flow.
Support and updates are provided through the booking workflow.