In association with Thyrocare NABL-Accredited Lab FMF-Certified Risk Assessment Tool Free Home Collection Pan-India Service
Double Marker (Dual Marker) Test by Thyrocare
The Double Marker test, also called the Dual Marker test, is a first-trimester prenatal blood screening used to estimate the risk of chromosomal conditions such as Down syndrome and Edwards syndrome. It measures PAPP-A and free β-hCG, and the result is interpreted with gestational age details and the NT scan report. This test is performed between the 8th and 13th weeks of pregnancy.
Free home sample collection for a dual marker test is available in 5,000+ area PIN code locations across India.
Double Marker Test Price, Sample, and Reporting Time
| Test name | Double Marker – First-trimester pregnancy screening |
| Other names of the test | Dual markers, First-trimester maternal serum screening |
| Test Type | First-trimester prenatal screening |
| Offer Price | ₹1,949 |
| What’s measured | Free β-hCG and Pregnancy-Associated Plasma Protein-A (PAPP-A); reported with MoM normalization |
| Testing Period | Between the 8th and 13th weeks of pregnancy |
| Sample type | Blood (serum) |
| Sample quantity | 4 ml |
| Fasting | Not required |
| Reporting time | around 48 hours after sample collection |
| Report format | Biomarker values (including MoM) and risk estimate output graph (in probability) |
| Testing Lab | Thyrocare |
| Lab Credentials | CAP-accredited, NABL-accredited, and ISO-certified |
| Test Quality | Uses FMF-certified biomarker testing kits and SSDW Lab 6 software for better accuracy |
What’s Included in the Double Marker (Dual Marker) Test
Biomarkers
PAPP-A and free β-hCG from a single blood sample.
Risk calculation
The biomarker values are interpreted with gestational age and relevant maternal details to generate the screening risk estimate.
Screening output graph
Provides a risk probability, such as high-risk, medium-risk, or low-risk.
Preparation for a Double Marker Test
- Fasting: Not required. Stay well-hydrated.
- Gestational age (GA): Share your last menstrual period (LMP) to calculate GA.
- Documents: Keep the test requisition form (TRF) and the NT scan report ready. These help confirm whether the required screening is a double marker, triple marker, or quadruple marker test.
- Clinical context: Mention all required context, including pregnancy type (twin, ART, or IVF), pregnancy history (number of children and miscarriage history, if any), and whether diabetic or not, so the lab pathway can be applied appropriately.
- Habits: Mention smoking or alcohol use, if any, because these details are relevant to risk interpretation.
- DOB: Provide the correct date of birth, because age is part of the risk calculation.
How to Book the Double Marker Test
- Send your city name or area PIN code on WhatsApp so we can confirm coverage and the earliest slot.
- Share the beneficiary’s name, age, gender, complete address, contact details, and preferred date and time of sample collection.
- Sample collection will be performed on time by a trained phlebotomist.
- Provide the required information (correct DOB, weight, pregnancy history and type, clinical history, and habits) and a copy of the NT scan report to the phlebotomist.
- Receive your report via email/WhatsApp within the expected turnaround time.
Book Now, Pay During the Sample Collection
Sample Report of the Double Marker Test
A sample FMF-certified Double Marker report graph is shown below.
To view a sample of the complete report, including biomarker values, the graph, and the final risk assessment, use the link below.
Why a Double Marker Test Is Advised in Pregnancy
The Double Marker test is used in the first trimester to screen for the risk of certain chromosomal conditions. It is advised, along with an NT scan, when early pregnancy risk assessment is needed.
This screening does not give a final diagnosis. It helps identify whether the pregnancy falls into a lower-risk or higher-risk category and whether further evaluation may be needed.
Limitations of the Double Marker Test
- The Double Marker test is a screening test; it does not confirm or rule out a chromosomal condition.
- In triplet pregnancy, the Double Marker risk assessment is not possible. In twin pregnancy, risk estimation may be less accurate.
- After 13 weeks of pregnancy, Double Marker risk estimation is not possible. In such a case, a triple marker or quadruple marker test is suggested, depending on the clinical context.
- Incorrect information in the test requisition form or patient details can affect risk calculation.
- If the report shows high risk, your obstetrician may advise genetic counselling, further screening with NIPT, or diagnostic testing such as CVS or amniocentesis.
Double Marker Test vs NIPT, Triple Marker, and Quadruple Marker Tests
These prenatal screening tests are performed at different stages of pregnancy. The test advised depends on the gestational age, previous screening results, ultrasound findings, and the complete pregnancy assessment.
| Test | Pregnancy period | Parameters or inputs | Screening purpose |
|---|---|---|---|
| Double Marker Test | First trimester, within the accepted testing period | PAPP-A and free β-hCG, interpreted with the NT scan and maternal details | Estimates the risk of chromosomal conditions such as Down syndrome and Edwards syndrome |
| NIPT | From 10 weeks of pregnancy | Cell-free DNA found in the maternal blood sample | Screens for common fetal chromosomal conditions, including trisomies 21, 18, and 13 |
| Triple Marker Test | Second trimester, generally between 15 and 22 weeks | AFP, hCG, and unconjugated estriol | Second-trimester screening for chromosomal conditions and neural tube defects |
| Quadruple Marker Test | Second trimester, generally between 15 and 22 weeks | AFP, hCG, unconjugated estriol, and inhibin A | Second-trimester screening using four maternal blood parameters |
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FAQs on Double Marker Test by Thyrocare
What is the price of the Double Marker test?
Is fasting required for the Double Marker test?
When is the Double Marker test performed?
Do I need an NT scan for Double Marker screening?
What does a high-risk result mean?
Can the Double Marker test confirm Down syndrome?
Which test is advised if the Double Marker test period is missed?
How soon will I receive my report?
Is home sample collection available across India?
Written by:
Hema Mehta Sahoo
Medically reviewed by:
Dr. M A Khan, MBBS, MD Pathology
Last updated: June 24, 2026
References
- Fetal Medicine Foundation: Screening for fetal aneuploidies at 11–13 weeks using maternal age, NT, free β-hCG, and PAPP-A.
- ACOG: First-trimester prenatal genetic screening with a maternal blood test and nuchal translucency ultrasound.
- ACOG: Current guidance on prenatal genetic screening, NIPT, CVS, and amniocentesis.