ANA Test

What does the ANA test detect?

The ANA test looks for autoantibodies that bind to nuclear components. A positive result suggests immune dysregulation and warrants clinical correlation. Pattern awareness (homogeneous, speckled, nucleolar, centromere) guides differential diagnosis and reflex testing such as ANA/ENA blot (ANA Profile).

Test Method & Reference Range

• Method: E.L.I.S.A (Enzyme-Linked Immunosorbent Assay)
• Technology: Indirect solid-phase immunoassay
• Unit: AU/mL
• Reference Range:
  • Negative: ≤ 25 AU/mL
  • Positive: ≥ 25 AU/mL

This standardized approach supports consistent reporting and reproducibility for longitudinal care.

Key Specifications

• Sensitivity: 87.1%
• Specificity: 80%
• Precision:
  • Intra-assay (%CV): ≤ 6.6
  • Inter-assay (%CV): ≤ 13.3
• Kit validation reference: The Lancet, September 15, 1984: 611–13

Consider testing if you have persistent, unexplained symptoms:

• Recurring fever without infection
• Rashes or skin-pigment changes
• Fatigue unresponsive to rest
• Joint pain, stiffness, or swelling
• Muscle aches or weakness
• Unexplained chest pain

Chronic or clustered symptoms should be reviewed with your physician, who may advise ANA testing and targeted follow-ups.

Autoimmunity results from misdirected immune recognition of “self”. ANA positivity is relevant to:

• Systemic Lupus Erythematosus (SLE)
• Sjogren syndrome
• Scleroderma
• Mixed connective-tissue disease (MCTD)
• Rheumatoid arthritis (specific patterns)

The test supports initial screening, prognosis, and treatment monitoring when combined with clinical findings.

You can access reliable ANA test services across top Indian cities. City-specific offers and ANA test price are shown at checkout.