Thyrocare’s ANA/ENA Blot Profile
Book Thyrocare’s comprehensive ANA Blot Profile (ENA) panel across India. Reports include 18 nuclear and cytoplasmic antigens to support autoimmune evaluations.
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ANA Blot Profile (ENA) Test: Quick Facts
| Also Known As | ANA 17/18 Blot, ENA Blot Testing, Extractable Nuclear Antigen Panel, Comprehensive ANA Panel Testing. |
| Discounted Price | ₹3,499 (Flat 50% off on MRP: |
| Sample Type | Blood (Serum) |
| Fasting | Not required |
| Testing Lab | Thyrocare (NABL & CAP Accredited) |
| Clinical Use | Follow-up to positive ANA IFA; helps identify specific autoantibodies for conditions like SLE, Sjögren’s, etc. |
| Reporting Time | 36 to 48 hours after sample collection |
| Service Type | Free home sample collection (in 2,500+ PIN code areas in India). |
What Is an ANA/ENA Profile?
An ANA Profile (often referred to as an ENA Panel or ANA Blot Test) is an advanced blood test used as part of autoimmune disease evaluation. In autoimmune disorders, the immune system mistakenly creates autoantibodies that attack the body’s own healthy tissues.
While a basic screening test will tell a doctor that autoantibodies are present, the ANA Profile acts as a detailed follow-up. It breaks down the blood sample to identify exactly which specific nuclear or cytoplasmic antigens (proteins) the antibodies are attacking. Identifying the specific marker helps rheumatologists and clinicians differentiate between complex, overlapping conditions such as Lupus, Sjögren’s syndrome, Scleroderma, and Mixed Connective Tissue Disease (MCTD).
ANA Test vs. ANA Profile (ENA Blot)
Autoimmunity related to Antinuclear Antibodies (ANA) can be evaluated through different levels of testing and methods, including a simple ANA blood test, ANA Titer (ANA by IFA), and ANA Profile (ANA/ENA Blot)
- ANA Test (Screening): At Thyrocare, the Antinuclear Antibodies (ANA) test is performed using a fully automated Indirect Solid Phase Immunoassay method. It answers a simple question: Are autoimmune antibodies present? It provides quantitative results in AU/mL units, but does not identify the specific antibody.
- ANA Titer Test or ANA by IFA (Second Level): Done by Thyrocare via fully automated Indirect IFA (Immunofluorescence Assay) to confirm the presence of autoimmune antibodies. It provides a positive/negative result, or a titer (concentration level, e.g., 1:80, 1:320) for positive cases, but does not identify the specific antibody.
- ANA Profile (ENA Blot Panel): This test panel extracts 18 autoimmune antibodies via Immunoblot technology. It answers a complex question: Which specific antibodies are present? It is often ordered after a positive screening test to provide more specific antibody information for clinical correlation.
Basic vs. Comprehensive ANA/ENA Profiles
Not all ANA Profiles are the same; labs offer different variations based on the number of antigens tested.
- Basic 6 to 7 Antinuclear Antibody Panel: Checks only the most common antibodies like SS-A, SS-B, Sm, RNP, Scl-70, ds-DNA, and Jo-1. A smaller panel can be useful for common antibody screening, but it may not cover less common markers included in broader 18-marker ANA/ENA profiles.
- Advanced 17 to 18 Marker Panel (ANA 17/18 Blot): It includes all basic autoimmune antibodies plus rare ones (like DFS70, Ku, Mi-2, AMA-M2, Ribosomal P). Testing rare markers is crucial for diagnosing overlapping syndromes or ruling out severe disease (e.g., isolated DFS70 is often benign).
An ANA/ENA panel with 6 to 8 antigen markers covers only selected antibodies. The 18-parameter ANA Blot Profile includes the common markers along with additional antibodies such as DFS70, Ku, Mi-2, AMA-M2, Ribosomal P, PM-Scl, and PCNA. This makes it useful for a broader review of autoimmune antibodies.
Parameters (Antigens) in the ANA Blot Profile
The Thyrocare ANA Profile (ENA 18 Blot Panel) tests for 18 specific nuclear and cytoplasmic antigens. Identifying these specific autoantibodies helps physicians confirm, rule out, or differentiate between various Systemic Autoimmune Rheumatic Diseases (SARD).
List of 18 Markers Included
- dsDNA
- Nucleosomes
- Histones
- SmD1
- U1-snRNP
- SS-A (Ro60)
- SS-A (Ro52)
- SS-B (La)
- Scl-70
- CENP-B
- Jo-1
- Ribosomal P-Protein
- PM-Scl
- PCNA
- AMA-M2
- DFS70
- Mi-2
- Ku
Clinical Significance of ANA Profile Antigens
The Thyrocare ANA/ENA Profile (Blot) tests for 18 different antigens. The presence of specific autoantibodies helps physicians point toward specific Systemic Autoimmune Rheumatic Diseases (SARD).
| Specific Marker(s) | Common Clinical Association / Significance |
|---|---|
| dsDNA, SmD1, Nucleosomes | Strongly associated with Systemic Lupus Erythematosus (SLE), especially when interpreted with symptoms and other reports. dsDNA levels can also correlate with disease activity and lupus nephritis (kidney involvement). |
| SS-A (Ro60 & Ro52), SS-B (La) | Strongly associated with Sjögren’s Syndrome (dry eyes/mouth) and SLE. Also vital for pregnant women, as these can carry a risk of neonatal lupus. |
| Scl-70, CENP-B | Markers for Systemic Sclerosis (Scleroderma). Scl-70 is linked to the diffuse severe form, while CENP-B is linked to the limited (CREST) syndrome. |
| U1-snRNP | A key marker considered in Mixed Connective Tissue Disease (MCTD) evaluation, a condition with overlapping features of lupus, scleroderma, and myositis. |
| Jo-1, Mi-2, Ku, PM-Scl | Associated with idiopathic inflammatory myopathies, such as Polymyositis and Dermatomyositis (muscle weakness and skin rashes). |
| AMA-M2 | Highly specific for Primary Biliary Cholangitis (PBC), an autoimmune disease of the liver. |
| DFS70 | A unique marker. If tested positive in isolation, without other disease-specific markers, it can reduce the likelihood of severe systemic autoimmune rheumatic disease. |
| Ribosomal P-Protein, Histones, PCNA | Further specific markers for SLE variants (e.g., Histones are linked to Drug-Induced Lupus, Ribosomal P to neuropsychiatric lupus). |
Note: The presence of an antibody does not guarantee the disease, and absence does not completely rule it out. Interpretation requires clinical correlation by a rheumatologist.
How ANA Profile or ENA 18 Blot Panel Test is Performed?
The ANA Profile is an advanced blood test that is performed from a 3 mL blood sample. At Thyrocare, the process of testing is as follows:
- A trained and experienced technician collects 3 mL of venous blood sample.
- Serum is separated from the blood by centrifuging the sample.
- The serum is transported to an advanced Thyrocare lab through cold-chain logistics.
- At the lab, the sample is processed using a HELMED machine, membrane-bound enzyme immunoassay (membrane filtration EIA) method, and immunoblot technology.
- The processing takes 30.5 hours at the lab.
- The report is verified by two MD pathologists and released.
How to Book an ANA Profile/Panel
- Message +91 70701 77147 on WhatsApp.
- Share the beneficiary’s name, age, gender, complete address with area PIN code, email ID, phone number, and preferred time slot.
- The booking team will confirm the order within a few minutes.
- A trained phlebotomist from Thyrocare will visit your home for sample collection.
- You will receive the digital report securely via WhatsApp and email in 36-48 hours.
ANA Profile Test: Sample Report
Sample reports are for format understanding only. Actual patient values are mapped against reference ranges and must be interpreted by a clinician.
FAQs — ANA Profile (ANA Panel) Test
What is an ANA Profile (ENA Panel) test?
How many parameters are included in the ANA Blot Profile?
Is ANA Profile the same as ENA Panel?
When is an ANA/ENA Blot test profile recommended?
References: MedlinePlus (ANA Test) • American College of Rheumatology
Note: This information is for educational and transactional purposes only. Always consult your physician to correlate the ANA Blot test result with your clinical symptoms and history.
Written by: Hema Mehta Sahoo
Medically reviewed by: Dr. M A Khan, MBBS, MD Pathology
Last updated: May 15, 2026