What is an ANA profile test?

The ANA Profile Test (Antinuclear Antibody Profile) is a comprehensive blood test used to detect the presence of antinuclear antibodies (ANAs)—immune proteins that mistakenly attack the nucleus of the body’s healthy cells. This test plays a crucial role in diagnosing a wide range of autoimmune disorders, such as lupus, Sjögren’s syndrome, scleroderma, and mixed connective tissue disease.Unlike a standard ANA test, the ANA profile offers a detailed evaluation by screening for 17 to 18 specific extractable nuclear antigens (ENAs). These are presented in a graphical blot format, allowing for precise identification of which autoantibodies are present. This detailed insight helps clinicians confirm, rule out, or differentiate between various autoimmune conditions with higher accuracy.

Is the ENA Test the same as the ANA Profile Test?

Yes, the ENA Panel and the ANA Profile Test refer to the same diagnostic test panel, commonly used to detect autoimmune disorders. These terms are often used interchangeably.In fact, this panel may also be labeled under different names, including:1. ANA 17 Blot2. Antinuclear Antibodies 17 Pro3. Extractable Nuclear Antigen (ENA) Profile4. Autoantibody Profile TestAll these names describe a single advanced test that screens for 17 to 18 specific autoantibodies targeting components within the nucleus of healthy cells. It’s a valuable tool for identifying autoimmune diseases such as lupus, scleroderma, and Sjögren’s syndrome.

What is the difference between an ANA test and an ANA profile test?

The ANA Test (Antinuclear Antibody Test) is a basic screening blood test that detects the presence of antinuclear antibodies (ANAs) in the blood. These antibodies are typically produced when the immune system mistakenly targets the nuclei of healthy cells, which can indicate an autoimmune condition.In contrast, the ANA Profile Test (also called the ENA Panel) is a more detailed and specific diagnostic test. It identifies individual extractable nuclear antigens (ENAs)—a group of 17 to 18 specific autoantibodies linked to various autoimmune diseases such as lupus, Sjögren’s syndrome, and mixed connective tissue disorders.While a positive ANA test suggests the possibility of an autoimmune disorder, the ANA Profile Test is typically recommended as a follow-up to pinpoint exactly which antibodies are involved and to help accurately diagnose the specific autoimmune condition.

Are the RIANA (ANA by IFA) test and ANA Profile test the same?

No, the RIANA Test (ANA by IFA) and the ANA Profile Test (ENA Panel) are not the same, although both are used to detect autoimmune disorders.The ANA by IFA (Indirect Immunofluorescence Assay), also known as the ANA Titer Test, is a screening test that detects the presence and pattern of total antinuclear antibodies in the blood. It gives a titer value and fluorescent pattern (e.g., homogenous, speckled, nucleolar), which helps indicate if autoimmunity may be present.On the other hand, the ANA Profile Test (or ENA Panel) is a more advanced and specific test that identifies individual extractable nuclear antigens (ENAs)—autoantibodies associated with specific autoimmune diseases. It provides detailed insights into the exact type of autoimmune disorder, such as lupus, Sjögren’s syndrome, or scleroderma.In short:1. ANA by IFA = Broad screening for ANA presence.2. ANA Profile/ENA Panel = Detailed detection of specific autoantibodies for accurate diagnosis.These tests are often used together, where the ANA IFA serves as an initial screen, and the ANA Profile helps confirm and differentiate specific autoimmune conditions.

When Is an ANA Profile Test Recommended?

An ANA Profile Test (ENA Blood Test Panel) is typically recommended after a positive ANA test result. If your initial ANA (Antinuclear Antibody) test indicates the presence of autoantibodies, your doctor may advise an ENA (Extractable Nuclear Antigen) panel—also known as the ANA Profile Test—for a more detailed diagnosis.This test helps to:1. Identify specific autoantibodies linked to autoimmune diseases like lupus, Sjögren’s syndrome, scleroderma, and mixed connective tissue disorder.2. Differentiate between autoimmune conditions that share similar symptoms.3. Guide your doctor in developing a personalized treatment plan.In short, if your ANA test is positive and you’re experiencing chronic fatigue, joint pain, rashes, or other autoimmune symptoms, your doctor may use the ENA/ANA profile test to pinpoint the exact cause.

Which autoimmune disorders can be detected by an ANA profie test?

The ANA (Antinuclear Antibody) or ENA (Extractable Nuclear Antigen) Profile Test is a powerful diagnostic tool used to detect a wide range of autoimmune disorders. These tests help identify specific autoantibodies targeting the body’sfor detecting healthy cells, enabling early and accurate diagnosis.Some of the common autoimmune diseases that can be detected through an ANA or ENA profile include:1. Systemic Lupus Erythematosus (SLE)2. Rheumatoid Arthritis (RA)3. Sjögren’s Syndrome4. Scleroderma (Systemic Sclerosis)5. Dermatomyositis6. Polymyositis7. Mixed Connective Tissue Disease (MCTD)8. Autoimmune Hepatitis9. CREST Syndrome

What should I do if my ANA/ENA profile report is positive?

If your ENA or ANA profile test report shows a positive result—meaning one or more antinuclear antibodies (ANAs) are detected—it’s essential to consult a qualified healthcare professional or a rheumatologist without delay.A positive result does not always confirm an autoimmune disease, but it indicates that your immune system may be attacking healthy tissues, which requires further evaluation. Your doctor will:1. Review your full medical history and symptoms2. Assess the specific antibodies identified in the report3. Possibly recommend additional diagnostic tests4. Determine if you meet the criteria for conditions like lupus, rheumatoid arthritis, Sjögren’s syndrome, or others5. Create a personalized treatment or monitoring plan, if needed6. Timely consultation and early intervention can significantly improve management and outcomes in autoimmune conditions. Never self-diagnose based on lab reports—always seek professional guidance.

How much does an ANA profile test cost?

The cost of an ANA Profile Test (also known as the ENA Blot Test) in India can vary based on several factors, including:1. Number of parameters tested (e.g., 17 or 18 ENA markers)2. Diagnostic lab reputation and certification3. Location of the testing center4. Service platform or healthcare providerOn average, the price ranges between ₹3,499 and ₹7,999 across India. Some diagnostic centers may charge more depending on the average monthly volume of samples they process. Labs receiving a lower volume of samples will charge higher to manage their operating costHowever, at Healthcare Offers, in collaboration with our trusted lab partner Thyrocare, we offer the ANA Profile Test at just ₹3,499 — with free home sample collection and quick report delivery.

Antinuclear Antibody Profile (ANA/ENA Panel) Test at ₹3,499

ANA Profile Test with 18 Blot at ₹3,499

Antinuclear Antibodies and Factors – 18 Extractable Nuclear Antigen (ENA) Test in a Single Profile

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ANA Profile Test by Thyrocare: Parameters, Price, and Panel Details

Blood Test Profile Name:	ANA Profile Test (Antinuclear Antibody Blot Profile)
Test Profile Other Names:	ANA Profile, Complete ANA Testing Profile, ANA 17 Blot Test, ANA 17 Profile, Antinuclear Antibodies Panel, Anti-Nuclear Antibodies 18 Tests, Fluorescent antinuclear antibody Panel, FANA, ANA Reflexive Panel, Full Antinuclear Antibody Profile, Extractable Nuclear Antigens Test (ENA Test), ENA Panel, ENA Profile Test, ENA 18 Blot, ENA 18 Antigens Profile, ANF Profile, Antinuclear Factor Panel, etc.
ANA Profile Significance:	It’s onfirmatory test for differential diagnosis of Systemic Rheumatic Diseases
No. of Parameters:	18
Offer Price:	₹3,499
Discount Applied:	50% on MRP ₹7,000 (Flat 50% off on ANA Profile Test Price)
Testing Lab:	Thyrocare
Lab Standard:	100% NABL-accredited with ISO and CAP certification. Fully automated to ensure reliable reports.
Fasting Required:	No
Other Preparation Required:	No
Reporting TAT:	Around 48 hours
Sample Type:	Blood (Serum in plain vial or gel tube)
Sample Quantity:	3 ml
Testing Technology:	Immunoblot using a fully automated Hemled machine
Testing Methodology:	Membrane-bound enzyme immunoassay
Available in:	All major cities in India (The ANA Profile Test is available in 200+ cities in India)
Service Type:	Service at home
Home Collection Charge:	₹0 (Free)
How to get service:	You can book your order over WhatsApp to get service at your convenience
How Can I Get the Reports:	You’ll get report links via email, SMS, and WhatsApp once the reports are ready

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About the ANA Profile Test

ANA Profile: An Overview

An Antinuclear Antibody (ANA) Profile or Extractable Nuclear Antigens (ENA) Test Profile has many other names. This advanced blood test profile looks for certain antibodies against some antigens found in the nucleus of a cell to detect and differentiate 17 to 18 autoimmune disorders through a western Blot method. The main purpose of the tests is to diagnose autoimmunity associated with Rheumatoid Arthritis, Sjögren’s Syndrome, Systemic Lupus Erythematosus (SLE), Mixed Connective Tissue Disease (MCTD), and some similar conditions.

An ENA/ANA profile test by Thyrocare is an accurate and reliable diagnosis for autoimmune disorders, including rhetamoid arthritis.

ANA Profile Test List

An ENA or ANA Profile Test includes the following 17 to 18 parameters:

DsDNA
Nucleosome
HIstone
SmD1
PCNa
P0 (RPP)
SS – A/Ro60
SS – A/Ro52
SS – B/La
CENBP
Scl70
U1 – SnRNP
AMA M2
Jo – 1
PM – Scl
Mi – 2
Ku
DFS – 70

ANA Profile Test (Anti-ENA Screen Test) Report

Here is a sample report of a typical ANA Profile (or Anti-ENA Profile):

ANA Profile Test (ENA Screen Test) by Thyrocare – Downloadable Sample Report

ANA Profile – ENA Blood Test Panel Download

ANA Profile Test (Anti-ENA Screening 17 to 18 Blot Tests) – Full Report Explanation

The ANA Profile Test (for 17 or 18 extractable nuclear antigens) is a specialized blood test designed to detect antinuclear antibodies (ANAs) by extracting nuclear antigens (ENAs) that are commonly associated with autoimmune diseases, especially rheumatoid arthritis. This complete and comprehensive panel helps identify the specific type of autoimmune condition by testing for multiple antibodies at once.

What Does an ANA Profile Test Measure?

An ANA Profile Test examines 17 to 18 ENAs by Western Blot Method to screen for autoantibodies against specific nuclear antigens, including:

dsDNA, Nucleosome, Histone – associated with Systemic Lupus Erythematosus (SLE)
SS-A/Ro60, SS-A/Ro52, SS-B/La – common markers of Sjögren’s Syndrome, SLE
Scl-70 – linked to Systemic Sclerosis (Scleroderma)
SmD1 – specifies SLE
Jo-1 – specified with Polymyositis and Dermatomyositis
CENP-B – a marker of Limited Cutaneous Systemic Sclerosis
U1-snRNP, PCNA, Ku, PM-Scl, Mi-2, DFS70, etc associated with RA

Each of these markers helps clinicians diagnose and differentiate between autoimmune disorders, as well as rhematoid factors.

ANA Profile Result Interpretation

Negative Result

If your ANA Profile Test is negative, it means no significant autoantibodies were detected:

Interpretation: Autoimmune diseases (especially Rheumatoid Arthritis and similar conditions) are unlikely.
Next Steps: If you are asymptomatic, no further autoimmune testing may be needed.
Important: In rare cases, autoimmune conditions may still be present even if ANA factors are negative (especially in early stages).

Positive Result

A positive ANA Profile Test indicates that your immune system is producing antibodies that may be attacking your body’s own tissues.

Interpretation: May suggest the presence of an autoimmune disease such as:
- Rhetamoid Arthritis
- SLE
- Sjögren’s Syndrome
- Scleroderma
- Mixed Connective Tissue Disease
- Polymyositis / Dermatomyositis
What to Expect:
- Your doctor may recommend further evaluation.
- Diagnosis is made based on symptoms, history, and other diagnostic findings—not the ANA test alone.

Frequently Asked Questions (FAQs) – ENA/ANA 17 Profile Test

As the ANA test profile has many different names and there are many different blood tests with similar names, confusion may happen. Here are answers to a few frequently asked questions that can clear the confusion.

What is an ANA profile test?
The ANA Profile Test (Antinuclear Antibody Profile) is a comprehensive blood test used to detect the presence of antinuclear antibodies (ANAs)—immune proteins that mistakenly attack the nucleus of the body’s healthy cells. This test plays a crucial role in diagnosing a wide range of autoimmune disorders, such as lupus, Sjögren’s syndrome, scleroderma, and mixed connective tissue disease.
Unlike a standard ANA test, the ANA profile offers a detailed evaluation by screening for 17 to 18 specific extractable nuclear antigens (ENAs). These are presented in a graphical blot format, allowing for precise identification of which autoantibodies are present. This detailed insight helps clinicians confirm, rule out, or differentiate between various autoimmune conditions with higher accuracy.
Is the ENA Test the same as the ANA Profile Test?
Yes, the ENA Panel and the ANA Profile Test refer to the same diagnostic test panel, commonly used to detect autoimmune disorders. These terms are often used interchangeably.
In fact, this panel may also be labeled under different names, including:
1. ANA 17 Blot
2. Antinuclear Antibodies 17 Pro
3. Extractable Nuclear Antigen (ENA) Profile
4. Autoantibody Profile Test
All these names describe a single advanced test that screens for 17 to 18 specific autoantibodies targeting components within the nucleus of healthy cells. It’s a valuable tool for identifying autoimmune diseases such as lupus, scleroderma, and Sjögren’s syndrome.
What is the difference between an ANA test and an ANA profile test?
The ANA Test (Antinuclear Antibody Test) is a basic screening blood test that detects the presence of antinuclear antibodies (ANAs) in the blood. These antibodies are typically produced when the immune system mistakenly targets the nuclei of healthy cells, which can indicate an autoimmune condition.
In contrast, the ANA Profile Test (also called the ENA Panel) is a more detailed and specific diagnostic test. It identifies individual extractable nuclear antigens (ENAs)—a group of 17 to 18 specific autoantibodies linked to various autoimmune diseases such as lupus, Sjögren’s syndrome, and mixed connective tissue disorders.
While a positive ANA test suggests the possibility of an autoimmune disorder, the ANA Profile Test is typically recommended as a follow-up to pinpoint exactly which antibodies are involved and to help accurately diagnose the specific autoimmune condition.
Are the RIANA (ANA by IFA) test and ANA Profile test the same?
No, the RIANA Test (ANA by IFA) and the ANA Profile Test (ENA Panel) are not the same, although both are used to detect autoimmune disorders.
The ANA by IFA (Indirect Immunofluorescence Assay), also known as the ANA Titer Test, is a screening test that detects the presence and pattern of total antinuclear antibodies in the blood. It gives a titer value and fluorescent pattern (e.g., homogenous, speckled, nucleolar), which helps indicate if autoimmunity may be present.
On the other hand, the ANA Profile Test (or ENA Panel) is a more advanced and specific test that identifies individual extractable nuclear antigens (ENAs)—autoantibodies associated with specific autoimmune diseases. It provides detailed insights into the exact type of autoimmune disorder, such as lupus, Sjögren’s syndrome, or scleroderma.
In short:
1. ANA by IFA = Broad screening for ANA presence.
2. ANA Profile/ENA Panel = Detailed detection of specific autoantibodies for accurate diagnosis.
These tests are often used together, where the ANA IFA serves as an initial screen, and the ANA Profile helps confirm and differentiate specific autoimmune conditions.
When Is an ANA Profile Test Recommended?
An ANA Profile Test (ENA Blood Test Panel) is typically recommended after a positive ANA test result. If your initial ANA (Antinuclear Antibody) test indicates the presence of autoantibodies, your doctor may advise an ENA (Extractable Nuclear Antigen) panel—also known as the ANA Profile Test—for a more detailed diagnosis.
This test helps to:
1. Identify specific autoantibodies linked to autoimmune diseases like lupus, Sjögren’s syndrome, scleroderma, and mixed connective tissue disorder.
2. Differentiate between autoimmune conditions that share similar symptoms.
3. Guide your doctor in developing a personalized treatment plan.
In short, if your ANA test is positive and you’re experiencing chronic fatigue, joint pain, rashes, or other autoimmune symptoms, your doctor may use the ENA/ANA profile test to pinpoint the exact cause.
Which autoimmune disorders can be detected by an ANA profie test?
The ANA (Antinuclear Antibody) or ENA (Extractable Nuclear Antigen) Profile Test is a powerful diagnostic tool used to detect a wide range of autoimmune disorders. These tests help identify specific autoantibodies targeting the body’sfor detecting healthy cells, enabling early and accurate diagnosis.
Some of the common autoimmune diseases that can be detected through an ANA or ENA profile include:
1. Systemic Lupus Erythematosus (SLE)
2. Rheumatoid Arthritis (RA)
3. Sjögren’s Syndrome
4. Scleroderma (Systemic Sclerosis)
5. Dermatomyositis
6. Polymyositis
7. Mixed Connective Tissue Disease (MCTD)
8. Autoimmune Hepatitis
9. CREST Syndrome
What should I do if my ANA/ENA profile report is positive?
If your ENA or ANA profile test report shows a positive result—meaning one or more antinuclear antibodies (ANAs) are detected—it’s essential to consult a qualified healthcare professional or a rheumatologist without delay.
A positive result does not always confirm an autoimmune disease, but it indicates that your immune system may be attacking healthy tissues, which requires further evaluation. Your doctor will:
1. Review your full medical history and symptoms
2. Assess the specific antibodies identified in the report
3. Possibly recommend additional diagnostic tests
4. Determine if you meet the criteria for conditions like lupus, rheumatoid arthritis, Sjögren’s syndrome, or others
5. Create a personalized treatment or monitoring plan, if needed
6. Timely consultation and early intervention can significantly improve management and outcomes in autoimmune conditions. Never self-diagnose based on lab reports—always seek professional guidance.
How much does an ANA profile test cost?
The cost of an ANA Profile Test (also known as the ENA Blot Test) in India can vary based on several factors, including:
1. Number of parameters tested (e.g., 17 or 18 ENA markers)
2. Diagnostic lab reputation and certification
3. Location of the testing center
4. Service platform or healthcare provider
On average, the price ranges between ₹3,499 and ₹7,999 across India. Some diagnostic centers may charge more depending on the average monthly volume of samples they process. Labs receiving a lower volume of samples will charge higher to manage their operating cost
However, at Healthcare Offers, in collaboration with our trusted lab partner Thyrocare, we offer the ANA Profile Test at just ₹3,499 — with free home sample collection and quick report delivery.

Most Important Thing to Know Before Booking an ANA Profile Test

When it comes to ANA (Antinuclear Antibody) testing, the names of the tests can be confusing. Multiple test panels use similar names with minor differences in suffixes or prefixes—leading many people to mistakenly book the wrong test. This can delay accurate diagnosis and proper treatment.

Let’s clear the confusion so you can make the right decision:

1. ANA or ANF (ELISA Method)

If your test is named simply as ANA or ANF, it usually refers to a basic screening test done using the ELISA method.

Purpose: Detects the presence of antinuclear antibodies (ANA total)
Approximate Cost in India: ₹700 – ₹1,000
Limitations: Does not identify specific autoantibodies

2. ANA Titer, ANA by IFA, or RIANA (Immunofluorescence Method)

These names indicate a more advanced method, where ANA is measured using Indirect Immunofluorescence Assay (IFA).

Purpose: Measures the concentration (titer) and pattern of ANAs
Cost Range: Slightly higher than ELISA
Use Case: Often used as a follow-up to ELISA for confirmation

3. ANA Profile Test / ENA Test Panel / ANA 17 Blot Test

These refer to comprehensive autoantibody panels such as the ANA Profile 17, ENA Panel, or Extractable Nuclear Antigen (ENA) Profile.

Purpose: Identifies 17+ specific autoantibodies (like anti-Sm, anti-RNP, anti-SSA, etc.)
Also Known As: ANA 17 Profile, ENA Blot Test
Price Range: ₹3,499 – ₹7,999
Clinical Use: Detailed diagnosis for autoimmune diseases such as lupus, Sjögren’s syndrome, scleroderma, and MCTD

Quick Tip Before You Book

To avoid confusion and choose the right ANA test, always:

Confirm the method (ELISA, IFA, or Blot)
Understand your doctor’s recommendation
Know whether you need a screening test or a comprehensive autoimmune profile

At Healthcare Offers, we help you make the right choice with expert-backed test details and affordable lab rates in partnership with Thyrocare.

Other Relevant Autoimmunity Blood Tests

Test/Profile Name	No. of Parameters	Offer Price	MRP	Booking Option
Antinuclear Antibody, ANA, ANA by ELISA	01	₹779	₹1,000	WhatsApp to Book Now
RIANA – ANA by IFA – ANA Titre (Immunofluorescence)	01	₹799	₹1,100	WhatsApp to Book Now
Rheumatoid Arthritis Factor, RA Factor, RF	01	₹649	₹800	WhatsApp to Book Now
Anti-CCP (ACCP)	01	₹1,699	₹2,400	WhatsApp to Book Now
NK Cell (CD16 + CD56) Analysis (Immune Function Profile)	32	₹2,099	₹4,500	WhatsApp to Book Now
CD3+CD4+CD8	07	₹1,999	₹4,000	WhatsApp to Book Now
Immune Function Panel	32	₹2,099	₹4,500	WhatsApp to Book Now
HLA B27 (by Flowcytometry)	01	₹1,999	₹2,600	WhatsApp to Book Now
HLA B27 Real Time PCR, HLA-B27 PCR	01	₹2,199	₹3,600	WhatsApp to Book Now
ASO Titer Test	01	₹629	₹789	WhatsApp to Book Now
Basic Arthritis Profile	72	₹1,999	₹4,000	WhatsApp to Book Now
Advanced Arthritis Profile	76	₹3,199	₹6,000	WhatsApp to Book Now

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Trusted References on the ANA Profile Test

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ANA Profile Test at the Best Price: ENA Profile Test 17/18 Blot