ANA Profile Test by Thyrocare: Parameters, Price, and Panel Details
| Blood Test Profile Name: | ANA Profile Test (Antinuclear Antibody Blot Profile) |
| Test Profile Other Names: | ANA Profile, Complete ANA Testing Profile, ANA 17 Blot Test, ANA 17 Profile, Antinuclear Antibodies Panel, Anti-Nuclear Antibodies 18 Tests, Fluorescent Antinuclear Antibody Panel, FANA, ANA Reflexive Panel, Full Antinuclear Antibody Profile, Extractable Nuclear Antigens Test (ENA Test), ENA Panel, ENA Profile Test, ENA 18 Blot, ENA 18 Antigens Profile, ANF Profile, Antinuclear Factor Panel, etc. |
| ANA Profile Significance: | Helps in the differential diagnosis of systemic rheumatic diseases; results are interpreted alongside clinical findings[1][2][3] |
| No. of Parameters: | 18 |
| Offer Price: | ₹3,499 |
| Discount Applied: | 50% on MRP ₹7,000 (Flat 50% off) |
| Testing Lab: | Thyrocare |
| Lab Standard: | NABL-accredited with ISO and CAP certification; automated processing for reliable reports |
| Fasting Required: | No |
| Other Preparation Required: | No |
| Reporting TAT: | Around 48 hours |
| Sample Type: | Blood (serum in plain vial or gel tube) |
| Sample Quantity: | 3 ml |
| Testing Technology: | Immunoblot using a fully automated Hemled machine |
| Testing Methodology: | Membrane-bound enzyme immunoassay |
| Available in: | All major cities in India (available in 200+ cities) |
| Service Type: | Service at home |
| Home Collection Charge: | ₹0 (Free) |
| How to get service: | Book over WhatsApp at your convenience |
| How you’ll receive reports: | Report links via email, SMS, and WhatsApp |
About the ANA Profile Test
ANA Profile: An Overview
An Antinuclear Antibody (ANA) Profile — also called an Extractable Nuclear Antigens (ENA) Profile — looks for specific autoantibodies against antigens in the cell nucleus using an immunoblot (“blot”) method. By assessing 17–18 ENA targets in one panel, it helps doctors differentiate between autoimmune conditions such as Rheumatoid Arthritis (RA), Sjögren’s Syndrome, Systemic Lupus Erythematosus (SLE), and Mixed Connective Tissue Disease (MCTD). Results guide diagnosis when interpreted with symptoms and other investigations[1][3][5].
An ENA/ANA Profile test by Thyrocare delivers detailed antibody information that supports accurate clinical decision-making for autoimmune disorders.
ANA Profile Test List
This ENA/ANA Profile includes the following 17–18 parameters:
- dsDNA
- Nucleosome
- Histone
- SmD1
- PCNA
- P0 (RPP)
- SS-A/Ro60
- SS-A/Ro52
- SS-B/La
- CENP-B
- Scl-70
- U1-snRNP
- AMA-M2
- Jo-1
- PM-Scl
- Mi-2
- Ku
- DFS-70
ANA Profile (Anti-ENA) Sample Report
Here is a sample report of a typical ANA Profile (Anti-ENA Profile):
Downloadable Sample Report — ANA Profile (ENA Screen) by Thyrocare
ANA Profile — ENA Blood Test Panel • Download
ANA Profile (17–18 Blot) — What the Panel Measures
The panel screens autoantibodies against specific nuclear antigens using an immunoblot method. Key associations include:
- dsDNA, Nucleosome, Histone — often seen in Systemic Lupus Erythematosus (SLE)
- SS-A/Ro60, SS-A/Ro52, SS-B/La — Sjögren’s Syndrome, sometimes SLE
- Scl-70 — Systemic Sclerosis (Scleroderma)
- SmD1 — supports SLE
- Jo-1 — associated with Polymyositis / Dermatomyositis
- CENP-B — limited cutaneous systemic sclerosis (CREST)
- U1-snRNP, PCNA, Ku, PM-Scl, Mi-2, DFS70 — considered with clinical context, sometimes overlapping with RA features
Each marker helps clinicians narrow the differential diagnosis when combined with history, exam, and other labs.
Result Interpretation (General Guidance)
Negative Result
If your ANA Profile is negative, no significant autoantibodies were detected:
- Interpretation: Certain autoimmune diseases become less likely; clinical context still matters[1].
- Next steps: If asymptomatic, additional autoimmune testing may not be necessary.
- Note: Rarely, autoimmune conditions can be present despite negative results (early disease, assay limitations)[4][5].
Positive Result
A positive ANA Profile indicates autoantibodies that may target the body’s own tissues.
- Interpretation: May suggest an autoimmune disease such as:
- Rheumatoid Arthritis
- SLE
- Sjögren’s Syndrome
- Scleroderma
- Mixed Connective Tissue Disease
- Polymyositis / Dermatomyositis
- What to expect:
- Your doctor may correlate findings with symptoms and may advise additional tests.
- Diagnosis is clinical; lab tests support but do not replace medical evaluation[1][3].
ANA Testing Methods — Quick Comparison
| Method | Purpose | Strengths | Limitations |
|---|---|---|---|
| ANA by ELISA | Initial screening (antibody binding to antigens on plates)[1][4] | Automated, affordable[4] | Lower sensitivity vs IFA; may miss certain patterns[2][5] |
| ANA by IFA (HEp-2) | Gold-standard screening[2][5] | Detects broad patterns; high sensitivity[2][3] | Requires expert interpretation; more manual[5] |
| ANA Profile / ENA Blot | Follow-up for specific autoantibodies[1][4] | Identifies 15–18 specific autoantibodies; aids differential diagnosis[3][4] | Not for initial screening; interpretation can be complex[5] |
Frequently Asked Questions (FAQs) — ENA/ANA 17–18 Profile
ANA test names can look similar. These answers clear common confusion and help you pick the right test.
What is an ANA profile test?
The ANA Profile (Antinuclear Antibody Profile) detects a group of 17–18 ENA autoantibodies linked to autoimmune diseases. It’s typically reviewed by clinicians alongside symptoms and other findings to confirm, rule out, or differentiate conditions such as lupus, Sjögren’s syndrome, scleroderma, and MCTD[1][3].
Is the ENA Test the same as the ANA Profile Test?
Yes — ENA Panel and ANA Profile refer to the same comprehensive panel (a.k.a. ANA 17/18 Blot) that screens for disease-specific autoantibodies[1][4].
What is the difference between an ANA test and an ANA profile test?
The basic ANA test screens for total ANA; the ANA Profile/ENA Panel identifies which specific autoantibodies are present, guiding the differential diagnosis after screening[1][2][4].
Are the RIANA (ANA by IFA) test and ANA Profile test the same?
No. ANA by IFA is a screening method that reports titer and pattern; the ANA Profile/ENA Panel identifies specific ENA antibodies. They are often used together (screen → reflex)[2][5].
When is an ANA Profile recommended?
Typically after a positive ANA screen and compatible symptoms, to pinpoint disease-related antibodies and guide next steps[1][3][4].
Which autoimmune disorders can be detected?
The panel supports evaluation for SLE, RA, Sjögren’s Syndrome, Scleroderma, Dermatomyositis/Polymyositis, MCTD, among others — always interpreted clinically[1][3].
What should I do if my ANA/ENA profile is positive?
Consult your physician or a rheumatologist. A positive result guides — but does not by itself establish — diagnosis. Your doctor will correlate with history, exam, and additional tests as needed[1][3][5].
How much does an ANA profile test cost?
Across India, pricing varies by parameters and lab. Typical range: ₹3,499–₹7,999. Through Healthcare Offers with Thyrocare, the ANA Profile (18 Blot) is available at ₹3,499 with free home collection.
Before You Book: Pick the Right ANA Test
Test names can be confusing. Use this quick guide to choose the right one:
1) ANA or ANF (ELISA method)
- Purpose: Broad screening for total ANA
- Approximate Cost in India: ₹700–₹1,000
- Limitations: Does not identify specific antibodies
2) ANA Titer / ANA by IFA / RIANA
- Purpose: Measures ANA titer and pattern (e.g., homogeneous, speckled, nucleolar)
- Cost: Slightly higher than ELISA
- Use: Often used as confirmatory screening because of higher sensitivity[2][5]
3) ANA Profile / ENA Panel / ANA 17–18 Blot
- Purpose: Identifies 17+ specific ENA antibodies (e.g., anti-Sm, anti-RNP, anti-SSA)
- Price Range: ₹3,499–₹7,999
- Use: Detailed follow-up after screening to help differentiate autoimmune conditions[1][4]
Other Relevant Autoimmunity Blood Tests
| Test/Profile Name | No. of Parameters | Offer Price | MRP | Booking Option |
|---|---|---|---|---|
| Antinuclear Antibody, ANA, ANA by ELISA | 01 | ₹779 | ₹1,000 | WhatsApp to Book Now |
| RIANA — ANA by IFA — ANA Titre (Immunofluorescence) | 01 | ₹799 | ₹1,100 | WhatsApp to Book Now |
| Rheumatoid Arthritis Factor (RA/RF) | 01 | ₹649 | ₹800 | WhatsApp to Book Now |
| Anti-CCP (ACCP) | 01 | ₹1,699 | ₹2,400 | WhatsApp to Book Now |
| NK Cell (CD16 + CD56) Analysis (Immune Function Profile) | 32 | ₹2,099 | ₹4,500 | WhatsApp to Book Now |
| CD3+CD4+CD8 | 07 | ₹1,999 | ₹4,000 | WhatsApp to Book Now |
| Immune Function Panel | 32 | ₹2,099 | ₹4,500 | WhatsApp to Book Now |
| HLA-B27 (Flow Cytometry) | 01 | ₹1,999 | ₹2,600 | WhatsApp to Book Now |
| HLA-B27 (Real-Time PCR) | 01 | ₹2,199 | ₹3,600 | WhatsApp to Book Now |
| ASO Titer Test | 01 | ₹629 | ₹789 | WhatsApp to Book Now |
| Basic Arthritis Profile | 72 | ₹1,999 | ₹4,000 | WhatsApp to Book Now |
| Advanced Arthritis Profile | 76 | ₹3,199 | ₹6,000 | WhatsApp to Book Now |
Trusted References on the ANA Profile Test
- MedlinePlus — Antinuclear Antibody (ANA) Test
- American College of Rheumatology — Antinuclear Antibodies (ANA)
- Mayo Clinic — ENA Panel Blood Test
- LabCorp — Antinuclear Antibody Testing
- UNC School of Medicine — Decoding the ANA: A Guide to ANA Testing
Declaration: We are an authorized sales partner for the listed health checkup services. Brand/product names are used with permission. We present research-based information and keep pages updated to align with current guidance.